Detection and Differentiation of In Vitro-Spiked Bacteria by Real-Time PCR and Melting-Curve Analysis
نویسندگان
چکیده
منابع مشابه
Detection and differentiation of in vitro-spiked bacteria by real-time PCR and melting-curve analysis.
We introduce a consensus real-time PCR protocol for the detection of bacterial DNA from laboratory-prepared specimens such as water, urine, and plasma. This prototype detection system enables an exact Gram stain classification and, in particular, screening for specific species of 17 intensive care unit-relevant bacteria by means of fluorescence hybridization probes and melting-curve analysis in...
متن کاملEvaluation of DNA Melting Curve Analysis Real-Time PCR for Detection and Differentiation of Cryptosporidium Species
Cryptosporidium spp. are protozoan parasites that infect various vertebrate hosts. Although 10 species are recognized, at least five types have been found to infect human(1). Thus, the identification of Cryptosporidium spp. and genotypes is important for the assessment of public health issues and for tracking infection or contamination sources(2). Presently, the identification of Cryptosporidiu...
متن کاملsimultaneous detection and identification of hbv and htlv-i viruses by melting curve analysis ofmultiplex real-time pcr
background and objectives:hbv and htlv-i are life threatening infectious agents in patients who receive blood and blood products. although serological methods have been proved to be useful, detection of these viruses has remained a challengingissue due to the many obstacles. by the advent of nucleic acid testing methods, especially in multiplex format, more precise detection is possible.the obj...
متن کاملDetection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis.
Several real-time PCR procedures for the detection and genotyping of oocysts of Cryptosporidium parvum were evaluated. A 40-cycle amplification of a 157-bp fragment from the C. parvum beta-tubulin gene detected individual oocysts which were introduced into the reaction mixture by micromanipulation. SYBR Green I melting curve analysis was used to confirm the specificity of the method when DNA ex...
متن کاملDetection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR.
Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical. We developed a real-time fluorescence-based multiplex PCR for the detection of al...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2004
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.42.2.512-517.2004